钠离子/牛磺胆酸共转运蛋白抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
钠离子/牛磺胆酸共转运蛋白抗体

钠离子/牛磺胆酸共转运蛋白抗体

商家询价

产品名称： 钠离子/牛磺胆酸共转运蛋白抗体

英文名称： SLC10A1

产品编号： 1958

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： WB ELISA Flow-Cyt ICC IF

上海雅吉生物科技有限公司
  • 联系人 :
  • 地址 : 上海市闵行区元江路5500号第1幢5658室
  • 邮编 :
  • 所在区域 : 上海
  • 电话 : 158****3937 点击查看
  • 传真 : 点击查看
  • 邮箱 : yajikit@163.com
进入展台

 

中文名称 钠离子/牛磺胆酸共转运蛋白抗体
别    名 Na+/taurocholate Cotransporting Polypeptide; Na(+)/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na / bile acid cotransporter; Na / taurocholate transport protein; Na(+)/taurocholate transport protein; NTCP; NTCP1; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1; Slc10a1 protein; Slc10a1; SBACT; SLC10A1; NTCP_HUMAN.  
研究领域 免疫学  信号转导  转运蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,  (predicted: Mouse, Rat, Dog, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /test ICC=1:100-500 IF=1:100-500 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 38kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NTCP:21-120/349 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization(SLC10A2; MIM 601295), and the other being found in the basolateral membranes of hepatocytes (SLC10A1).[supplied by OMIM]

Function:
The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Liver and kidney

Similarity:
Belongs to the sodium:bile acid symporter family.

SWISS:
Q14973

Gene ID:
6554

Database links:

Entrez Gene: 6554 Human

Entrez Gene: 20493 Mouse

Entrez Gene: 24777 Rat

Omim: 182396 Human

SwissProt: Q14973 Human

SwissProt: O08705 Mouse

SwissProt: P26435 Rat

Unigene: 952 Human

Unigene: 341781 Mouse

Unigene: 9913 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

NTCP（钠离子/牛磺胆酸共转运蛋白）(钠离子-牛磺胆酸共转运多肽)Na+/taurocholate Cotransporting Polypeptide是 肝脏的胆汁酸盐吸收主要是钠离子依赖性转运子NTCP介导的，主要吸收牛磺胆酸，是肝细胞特有的一种转运载体，分布在肝细胞基侧膜在肝小叶和肝的腺泡表达较多。
有学者认为：NTCP是乙肝病毒的细胞表面受体分子，是HBV和HDV的功能受体，这些研究将有待于科研人员的进一步证实。
产品图片 Sample: K562 (human)cell Lysate at 40 ug
Primary: Anti- SLC10A1 (bs-1958R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 48 kD
Tissue/cell: Siha cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SLC10A1) Polyclonal Antibody, Unconjugated (bs-1958R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.Tissue/cell: HT1080 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SLC10A1) Polyclonal Antibody, Unconjugated (bs-1958R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.Blank control: 293T(blue).
Primary Antibody:Rabbit Anti-SLC10A1 antibody(bs-1958R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA(green);
Isotype Control Antibody: Rabbit IgG(orange) used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

​