WEE1 polyclonal antibody
产品名称： WEE1 polyclonal antibody
英文名称： WEE1 polyclonal antibody
产品编号： PAB3322
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of WEE1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human WEE1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Human wee1 kinase is directly transactivated by and increased in association with c-Fos/AP-1: rheumatoid synovial cells overexpressing these genes go into aberrant mitosis.
Kawasaki H, Komai K, Nakamura M, Yamamoto E, Ouyang Z, Nakashima T, Morisawa T, Hashiramoto A, Shiozawa K, Ishikawa H, Kurosaka M, Shiozawa S.Oncogene. 2003 Oct 9;22(44):6839-44.
- 2.
- Inhibition of proteasome-dependent degradation of Wee1 in G2-arrested Hep3B cells by TGF beta 1.
Hashimoto O, Ueno T, Kimura R, Ohtsubo M, Nakamura T, Koga H, Torimura T, Uchida S, Yamashita K, Sata M.Mol Carcinog. 2003 Apr;36(4):171-82.
- 3.
- Depletion of Wee-1 kinase is necessary for both human immunodeficiency virus type 1 Vpr- and gamma irradiation-induced apoptosis.
Yuan H, Xie YM, Chen IS.J Virol. 2003 Feb;77(3):2063-70.
- Applications
- Western Blot
- The WEE1 polyclonal antibody (Cat # PAB3322) is used in Western blot to detect WEE1 in Jurkat cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2) .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with WEE1 polyclonal antibody (Cat # PAB3322) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 7465
- Protein Accession#:
- P30291
- Gene Name:
- WEE1
- Gene Alias:
- DKFZp686I18166,FLJ16446,WEE1A,WEE1hu
- Gene Description:
- WEE1 homolog (S. pombe)
- Omim ID:
- 193525
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq
- Other Designations:
- WEE1+ homolog,wee1 tyrosine kinase,wee1-like protein kinase
- Interactome
- Gene Pathway