MAP2K6 polyclonal antibody
产品名称： MAP2K6 polyclonal antibody
英文名称： MAP2K6 polyclonal antibody
产品编号： PAB3176
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MAP2K6.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MAP2K6.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- p38 Mitogen-activated protein kinase-dependent and -independent signaling of mRNA stability of AU-rich element-containing transcripts.
Frevel MA, Bakheet T, Silva AM, Hissong JG, Khabar KS, Williams BR.Mol Cell Biol. 2003 Jan;23(2):425-36.
- 2.
- Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence.
Wang W, Chen JX, Liao R, Deng Q, Zhou JJ, Huang S, Sun P.Mol Cell Biol. 2002 May;22(10):3389-403.
- 3.
- The MKK6/p38 mitogen-activated protein kinase pathway is capable of inducing SOCS3 gene expression and inhibits IL-6-induced transcription.
Bode JG, Ludwig S, Freitas CA, Schaper F, Ruhl M, Melmed S, Heinrich PC, Haussinger D.Biol Chem. 2001 Oct;382(10):1447-53.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of MAP2K6 polyclonal antibody (Cat # PAB3176) in mouse brain tissue lysate. MAP2K6 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP2K6 polyclonal antibody (Cat # PAB3176) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 5608
- Protein Accession#:
- P52564
- Gene Name:
- MAP2K6
- Gene Alias:
- MAPKK6,MEK6,MKK6,PRKMK6,SAPKK3
- Gene Description:
- mitogen-activated protein kinase kinase 6
- Omim ID:
- 601254
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq
- Other Designations:
- protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)