BAD polyclonal antibody
产品名称： BAD polyclonal antibody
英文名称： BAD polyclonal antibody
产品编号： PAB2450
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of BAD.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 100-130 of human BAD.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cleavage of 14-3-3 protein by caspase-3 facilitates bad interaction with Bcl-x(L) during apoptosis.
Won J, Kim DY, La M, Kim D, Meadows GG, Joe CO.J Biol Chem. 2003 May 23;278(21):19347-51. Epub 2003 Mar 25.
- 2.
- Role of PI3-kinase-dependent Bad phosphorylation and altered transcription in cytokine-mediated neutrophil survival.
Cowburn AS, Cadwallader KA, Reed BJ, Farahi N, Chilvers ER.Blood. 2002 Oct 1;100(7):2607-16.
- 3.
- Inhibition of phosphorylation of BAD and Raf-1 by Akt sensitizes human ovarian cancer cells to paclitaxel.
Mabuchi S, Ohmichi M, Kimura A, Hisamoto K, Hayakawa J, Nishio Y, Adachi K, Takahashi K, Arimoto-Ishida E, Nakatsuji Y, Tasaka K, Murata Y.J Biol Chem. 2002 Sep 6;277(36):33490-500. Epub 2002 Jun 26.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of mouse bladder tissue lysate (35 ug/lane) with BAD polyclonal antibody (Cat # PAB2450).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the BAD polyclonal antibody (Cat # PAB2450) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 572
- Protein Accession#:
- NP_116784;Q92934
- Gene Name:
- BAD
- Gene Alias:
- BBC2,BCL2L8
- Gene Description:
- BCL2-associated agonist of cell death
- Omim ID:
- 603167
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq
- Other Designations:
- BCL-X/BCL-2 binding protein,BCL2-antagonist of cell death protein,BCL2-binding component 6,BCL2-binding protein
- Gene Pathway
- Acute myeloid leukemia
- Alzheimer's disease
- Amyotrophic lateral sclerosis (ALS)
- Apoptosis
- Chronic myeloid leukemia
- Colorectal cancer
- Endometrial cancer
- ErbB signaling pathway
- Focal adhesion
- Insulin signaling pathway
- Melanoma
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- VEGF signaling pathway