SRC (phospho Y215) polyclonal antibody
产品名称： SRC (phospho Y215) polyclonal antibody
英文名称： SRC (phospho Y215) polyclonal antibody
产品编号： PAB0612
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of SRC.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y215 of human SRC.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Autocrine CSF-1R activation promotes Src-dependent disruption of mammary epithelial architecture.
Wrobel CN, Debnath J, Lin E, Beausoleil S, Roussel MF, Brugge JS.J Cell Biol. 2004 Apr 26;165(2):263-73.
- 2.
- Tyrosine 981, a novel ret autophosphorylation site, binds c-Src to mediate neuronal survival.
Encinas M, Crowder RJ, Milbrandt J, Johnson EM Jr.J Biol Chem. 2004 Apr 30;279(18):18262-9. Epub 2004 Feb 6.
- 3.
- Activation of beta-catenin-TCF-mediated transcription by non-receptor tyrosine kinase v-Src.
Haraguchi K, Nishida A, Ishidate T, Akiyama T.Biochem Biophys Res Commun. 2004 Jan 23;313(4):841-4.
- Applications
- Western Blot (Cell lysate)
- FG Pancreatic Carcinoma Cell stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x).
SRC (phospho Y215) polyclonal antibody (Cat # PAB0612) was diluted 1 : 500 in 1% BSA/TBST and incubated Overnight at 4 °C.
After washing 3x 5 min. with TBST the blots were incubated with 1 : 5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at room temperature.
The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.
Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD
- Entrez GeneID:
- 6714
- Protein Accession#:
- NP_005408;P12931
- Gene Name:
- SRC
- Gene Alias:
- ASV,SRC1,c-SRC,p60-Src
- Gene Description:
- v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
- Omim ID:
- 190090
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000174476,OTTHUMP00000174477,proto-oncogene tyrosine-protein kinase SRC,protooncogene SRC, Rous sarcoma,tyrosine kinase pp60c-src,tyrosine-protein kinase SRC-1
- Related Disease