FITC标记的磷酸化DDX58抗体
产品名称： FITC标记的磷酸化DDX58抗体
英文名称： Anti-phospho-DDX58 (Ser8)/FITC
产品编号： HZ-5299R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-phospho-DDX58 (Ser8)/FITC Conjugated antibody
FITC标记的磷酸化DDX58抗体
| 英文名称 | Anti-phospho-DDX58 (Ser8)/FITC |
| 中文名称 | FITC标记的磷酸化DDX58抗体 |
| 别 名 | p-DDX58 (Ser8); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I; DKFZp434J1111; DKFZp686N19181; FLJ13599; C330021E21; OTTHUMP00000045225; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD (Asp Glu Ala Asp/His) box polypeptide; DEAD box protein 58; Probable ATP dependent RNA helicase DDX58; Retinoic acid inducible gene 1 protein; RIG I; rig-I; RIG1; rigi; RNA helicase. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 细胞凋亡 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 102kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human DDX58 around the phosphorylation site of Ser8 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG1/DDX58) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG1/DDX58 activates the kinases TBK1 and IKKe through the adaptor protein IPS1. These kinases then phosphorylate the transcription factors IRF3 and IRF7 which are essential for the expression of type-I interferons. RIG1/DDX58 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus. Function: Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. Subunit: Monomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58/LGP2, IKBKE, TBK1 and TMEM173/STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS to DDX58. Interacts with SRC. Subcellular Location: Cytoplasm. Note=Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Tissue Specificity: Present in vascular smooth cells (at protein level). Post-translational modifications: Phosphorylated in resting cells and dephosphorylated in RNA virus-infected cells. Phosphorylation at Thr-770, Ser-854 and Ser-855 results in inhibition of its activity while dephosphorylation at these sites results in its activation. Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation. Ubiquitinated. Undergoes 'Lys-48'- and 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS/IPS1 binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Also probably deubiquitinated by USP17L2/USP17 that cleaves 'Lys-48'-and 'Lys-63'-linked ubiquitin chains and positively regulates the receptor. Similarity: Belongs to the helicase family. Contains 2 CARD domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Database links: UniProtKB/Swiss-Prot: O95786.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
先天免疫系统通过识别各种病毒成分检测病毒感染并触发抗病毒反应。与Toll样受体3（TLR3）一样，胞质螺旋酶视黄酸诱导基因蛋白1（RIG1/DDX58）识别双链（DS）RNA，这是与病毒感染相关的分子模式。然而，与TLR3不同，RIG1/DDX58通过适配器蛋白IPS1激活激酶TBK1和IKKe。这些激酶然后磷酸化转录因子Irf3和Irf7，这对于I型干扰素的表达是必需的。RIG1/DDX58是针对干扰素的应答所需的，包括RNA病毒，包括副粘病毒、流感病毒和乙脑病毒。