水通道蛋白5抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
水通道蛋白5抗体

水通道蛋白5抗体

商家询价

产品名称： 水通道蛋白5抗体

英文名称： Aquaporin 5

产品编号： 0479

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： ELISA IHC-P IHC-F Flow-Cyt IF

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中文名称 水通道蛋白5抗体
别    名 Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5.  

 

研究领域 信号转导  通道蛋白  上皮细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Sheep, )
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 29kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse AQP5:201-265/265 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008]

Function:
Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:
Q9WTY4

Gene ID:
11830

Database links:

Entrez Gene: 362 Human

Entrez Gene: 11830 Mouse

Entrez Gene: 25241 Rat

Omim: 600442 Human

SwissProt: P55064 Human

SwissProt: Q9WTY4 Mouse

SwissProt: P47864 Rat

Unigene: 298023 Human

Unigene: 45580 Mouse

Unigene: 10066 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

水通道蛋白(Aquaporin,AQP)是一组与水分子通透有关的特异性细胞膜转运蛋白。其中,AQP 5在哺乳动物气管、支气管的上皮细胞表达较常见.
产品图片 Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 5) Polyclonal Antibody, Unconjugated (bs-1554R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Aquaporin 5 Polyclonal Antibody, Unconjugated(bs-1554R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela(blue).
Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1554R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Blank control: A549(blue).
Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.