MLF1IP polyclonal antibody
产品名称： MLF1IP polyclonal antibody
英文名称： MLF1IP polyclonal antibody
产品编号： PAB10268
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant MLF1IP.
- Immunogen:
- Recombinant protein corresponding to N-terminus 200 amino acids of human MLF1IP.
- Host:
- Rabbit
- Reactivity:
- Bovine, Chicken, Chimpanzee, Dog, Horse, Human, Mouse, Rat
- Specificity:
- This affinity purified antibody is directed against human MLF1IP protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:1400000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Regulation of myeloid leukemia factor-1 interacting protein (MLF1IP) expression in glioblastoma.
Hanissian SH, Teng B, Akbar U, Janjetovic Z, Zhou Q, Duntsch C, Robertson JH.Brain Res. 2005 Jun 14;1047(1):56-64.
- 2.
- cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP.
Hanissian SH, Akbar U, Teng B, Janjetovic Z, Hoffmann A, Hitzler JK, Iscove N, Hamre K, Du X, Tong Y, Mukatira S, Robertson JH, Morris SW.Oncogene. 2004 Apr 29;23(20):3700-7.
- 3.
- Identification of a novel cellular transcriptional repressor interacting with the latent nuclear antigen of Kaposi's sarcoma-associated herpesvirus.
Pan HY, Zhang YJ, Wang XP, Deng JH, Zhou FC, Gao SJ.J Virol. 2003 Sep;77(18):9758-68.
- Applications
- Western Blot (Cell lysate)
- Western blot using MLF1IP polyclonal antibody (Cat # PAB10268) shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of HeLa cells (- lane). Cells treated with MLF1IP shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1 : 1,000.
Personal Communication, K.S. Lee, NCI, Bethesda, MD.
- Entrez GeneID:
- 79682
- Protein Accession#:
- NP_078905;Q71F23
- Gene Name:
- MLF1IP
- Gene Alias:
- CENP-50,CENP-U,CENP-U(50),CENP50,CENPU,FLJ23468,KLIP1,PBIP1
- Gene Description:
- MLF1 interacting protein
- Omim ID:
- 611511
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
- Other Designations:
- KSHV latent nuclear antigen interacting protein 1,centromere protein U,polo-box-interacting protein 1